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Data sheet of POU5F1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chimpanzee,Dog,Horse,Human,Macaque,Mink,Mouse,Pig |
| Host species | Rabbit |
| Applications | WB,IP,ELISA,IF |
More info about POU5F1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10296 |
| Product name: | POU5F1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of POU5F1. |
| Gene id: | 5460 |
| Gene name: | POU5F1 |
| Gene alias: | MGC22487|OCT3|OCT4|OTF3|OTF4 |
| Gene description: | POU class 5 homeobox 1 |
| Immunogen: | A synthetic peptide corresponding to internal region of human POU5F1. |
| Protein accession: | NP_976034;Q01860 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:350000) Western Blot (1:1000-1:1500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody reacts with human and mouse Oct-4 protein. |
| Reactivity: | Bovine,Chimpanzee,Dog,Horse,Human,Macaque,Mink,Mouse,Pig |
| Application image: |  |
| Application image note: | Western blot using POU5F1 polyclonal antibody (Cat # PAB10296) shows detection of endogenous POU5F1 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kDa (arrowhead) corresponds to POU5F1. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1 : 1,000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with POU5F1 (lanes 3 and 4, respectively). |
| Applications: | WB,IP,ELISA,IF |
| Shipping condition: | Dry Ice |
| Publications: | High Oct-ane fuel powers the stem cell.Donovan PJ. Nat Genet. 2001 Nov;29(3):246-7. |