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Data sheet of NEDD4 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chimpanzee,Dog,Horse,Human,Macaque,Rabbit |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IF |
More info about NEDD4 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10283 |
| Product name: | NEDD4 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of NEDD4. |
| Gene id: | 4734 |
| Gene name: | NEDD4 |
| Gene alias: | KIAA0093|MGC176705|NEDD4-1|RPF1 |
| Gene description: | neural precursor cell expressed, developmentally down-regulated 4 |
| Immunogen: | A synthetic peptide corresponding to internal region of human NEDD4. |
| Protein accession: | NP_006145;P46934 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:20000-1:35000) Western Blot (1:300-1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody reacts with endogenous Nedd4 protein. |
| Reactivity: | Bovine,Chimpanzee,Dog,Horse,Human,Macaque,Rabbit |
| Application image: |  |
| Application image note: | Western blot using NEDD4 polyclonal antibody (Cat # PAB10283) shows detection of a 115 kDa band corresponding to endogenous NEDD4 (arrowhead) in MDA-MB-435 cell lysates. The blot was blocked with 5% BLOTTO overnight at 4°C. Primary antibody was used at a 1 : 350 dilution in 5% BLOTTO followed by reaction with a 1 : 20,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection. |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+.Dinudom A, Harvey KF, Komwatana P, Young JA, Kumar S, Cook DI. Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7169-73. |