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Data sheet of MTOR polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about MTOR polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10278 |
| Product name: | MTOR polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of MTOR. |
| Gene id: | 2475 |
| Gene name: | MTOR |
| Gene alias: | FRAP|FRAP1|FRAP2|RAFT1|RAPT1 |
| Gene description: | mechanistic target of rapamycin |
| Immunogen: | A synthetic peptide corresponding to amino acids 2440-2457 of human MTOR. |
| Protein accession: | P42345 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:20000) Western Blot (1:250-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from human derived tissues and cells. |
| Reactivity: | Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using MTOR polyclonal antibody (Cat # PAB10278) shows detection of a band ~ 245 KDa corresponding to human MTOR (arrowhead). Approximately 30 ug of HEK293 cell lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1 : 650 for 2h at RT. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.Nojima H, Tokunaga C, Eguchi S, Oshiro N, Hidayat S, Yoshino K, Hara K, Tanaka N, Avruch J, Yonezawa K. J Biol Chem. 2003 May 2;278(18):15461-4. Epub 2003 Feb 25. |