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Data sheet of LIMK1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human,Mouse,Orangutan,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ti,IF |
More info about LIMK1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10258 |
| Product name: | LIMK1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of LIMK1. |
| Gene id: | 3984 |
| Gene name: | LIMK1 |
| Gene alias: | LIMK |
| Gene description: | LIM domain kinase 1 |
| Immunogen: | A synthetic peptide corresponding to amino acids 630-647 of human LIMK1. |
| Protein accession: | P53667;NP_002305(isoform1) |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:8000-1:36000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity purified antibody is directed against human LIM kinase protein. |
| Reactivity: | Chimpanzee,Human,Mouse,Orangutan,Rat |
| Application image: |  |
| Application image note: | Western blot using LIMK1 polyclonal antibody (Cat # PAB10258) shows detection of a 73 KDa band corresponding to LIMK1 in lysates from mouse brain. Approximately 18 ug of lysate wasrun on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of LIMK1 polyclonal antibody. The doublet band at ~ 75 KDa may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 KDa is unknown. Signal was detected using standard techniques. |
| Applications: | ELISA,WB-Ti,IF |
| Shipping condition: | Dry Ice |
| Publications: | p57Kip2 regulates actin dynamics by binding and translocating LIM-kinase 1 to the nucleus.Yokoo T, Toyoshima H, Miura M, Wang Y, Iida KT, Suzuki H, Sone H, Shimano H, Gotoda T, Nishimori S, Tanaka K, Yamada N. J Biol Chem. 2003 Dec 26;278(52):52919-23. Epub 2003 Oct 6. |