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Data sheet of GGA1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about GGA1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10213 |
| Product name: | GGA1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of GGA1. |
| Gene id: | 26088 |
| Gene name: | GGA1 |
| Gene alias: | - |
| Gene description: | golgi associated, gamma adaptin ear containing, ARF binding protein 1 |
| Immunogen: | A synthetic peptide corresponding to amino acids 336-352 of human GGA1. |
| Protein accession: | Q9UJY5;NP_037497 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | The immunizing sequence is present only in isoform 1 of this protein, therefore cross reactivity with splice variants is not expected. |
| Reactivity: | Bovine,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using GGA1 polyclonal antibody (Cat # PAB10213) shows detection ofbands at ~100 KDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4°C followed by washes and reactionwith a 1 : 10,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The interaction of the human GGA1 GAT domain with rabaptin-5 is mediated by residues on its three-helix bundle.Zhai P, He X, Liu J, Wakeham N, Zhu G, Li G, Tang J, Zhang XC. Biochemistry. 2003 Dec 2;42(47):13901-8. |