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Data sheet of Cebpd polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about Cebpd polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10182 |
| Product name: | Cebpd polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant Cebpd. |
| Gene id: | 12609 |
| Gene name: | Cebpd |
| Gene alias: | - |
| Gene description: | CCAAT/enhancer binding protein (C/EBP), delta |
| Immunogen: | Recombinant protein corresponding to N-terminus of mouse Cebpd. |
| Protein accession: | NP_031705;Q00322 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:9000) Western Blot (1:1000-1:15000) Immunoprecipitation (0.8-1.5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | No cross-reactivity is observed with mouse C/EBPalpha or C/EBPbeta. |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | Western blot using Cebpd polyclonal antibody (Cat # PAB10182) shows detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. The blot shows staining as follows : untreated wild-type (WT) cells (lane 1), WT cells treated 3 h with LPS (lane 2) and Cebpd knock-out cells treated 3H with LPS (lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to endogenous Cebpd. Primary antibody was used at 1 : 15,000. ECL was used for detection. Personal communication, E.Sterneck, NCI, Frederick, MD. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Proteasome-mediated CCAAT/enhancer-binding protein delta (C/EBPdelta) degradation is ubiquitin-independent.Zhou S, Dewille JW. Biochem J. 2007 Jul 15;405(2):341-9. |