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Data sheet of Angpt1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | WB,IP,ELISA,IHC-P |
More info about Angpt1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10092 |
| Product name: | Angpt1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Angpt1. |
| Gene id: | 11600 |
| Gene name: | Angpt1 |
| Gene alias: | 1110046O21Rik|Ang-1|Ang1 |
| Gene description: | angiopoietin 1 |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to N-terminus of mouse Angpt1. |
| Protein accession: | NP_033770 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:800) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | Immunoblotting of Angpt1 polyclonal antibody (Cat # PAB10092) was used at a 1:500 dilution to detect mouse Angptl1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Angpt1 (clone 1-8) expressing cells. Lane 3 - mouse Angpt1 (clone 1-15) expressing cells. Lane 4 - mouse Angpt2 (clone 2-9) expressing cells. Approximately 20 ug of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development. |
| Applications: | WB,IP,ELISA,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.Suri C, Jones PF, Patan S, Bartunkova S, Maisonpierre PC, Davis S, Sato TN, Yancopoulos GD. Cell. 1996 Dec 27;87(7):1171-80. |