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Data sheet of Ankrd26 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA |
More info about Ankrd26 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10090 |
| Product name: | Ankrd26 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Ankrd26. |
| Gene id: | 232339 |
| Gene name: | Ankrd26 |
| Gene alias: | 5730521P14Rik|BC008111|mKIAA1074 |
| Gene description: | ankyrin repeat domain 26 |
| Immunogen: | A synthetic peptide corresponding to amino acids 1-15 of mouse Ankrd26. |
| Protein accession: | AAH47067;Q69ZS2 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:20000-1:80000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This affinity-purified antibody is directed against mouse Ankrd26 protein. |
| Reactivity: | Bovine,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Ankrd26 polyclonal antibody (Cat # PAB10090) shows detection of a band at ~81 KDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3MES cell lysate-40 ug, Lane 4 293T-Ankrd26 transfected cell lysate-20 ug, Lane 5 control 293T celllysate-20 ug, Lane 6 BSA-Ankrd26 conjugate-20ng, Lane 7 BSA-500 ng, Lane 8 BSA-100 ng, Lane 9 BSA-20 ng. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed withthe primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000. Sizeestimation was made by comparison to prestained MW markers as indicated. Personal Communication.Ira Pastan, NIH, CCR, Bethesda, MD. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |