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Data sheet of ING3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human,Orangutan,Rat |
| Host species | Goat |
| Applications | ELISA,WB-Tr |
More info about ING3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10081 |
| Product name: | ING3 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of ING3. |
| Gene id: | 54556 |
| Gene name: | ING3 |
| Gene alias: | Eaf4|FLJ20089|ING2|p47ING3 |
| Gene description: | inhibitor of growth family, member 3 |
| Immunogen: | A synthetic peptide corresponding to amino acids 294-304 of human ING3. |
| Protein accession: | Q9HC99;NP_061944(isoform1);AAG12172 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Dog,Human,Orangutan,Rat |
| Application image: |  |
| Application image note: | Western blot using ING3 polyclonal antibody (Cat # PAB10081) shows detection of a band at ~55 KDa corresponding to ING3 in RKO cells transfected with ING3 (Lane 2). Control RKO cells do not show detection of this specific band (lane1). The identity of the non-specific bands at 33 KDa and 20 KDa has not been determined. Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human coloncancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membranewas probed with the primary antibody diluted to1:1,000. Incubation was at 4°C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG[H&L] MXHu for 45 min at room temperature. IRDye™800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye isa trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Different HATS of the ING1 gene family.Feng X, Hara Y, Riabowol K. Trends Cell Biol. 2002 Nov;12(11):532-8. |