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Data sheet of IRS1 (phospho S307) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Dog,Human,Monkey,Mouse,Pig,Rat |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Ce |
More info about IRS1 (phospho S307) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10078 |
| Product name: | IRS1 (phospho S307) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1. |
| Gene id: | 3667 |
| Gene name: | IRS1 |
| Gene alias: | HIRS-1 |
| Gene description: | insulin receptor substrate 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S307 of human IRS1. |
| Protein accession: | P35568;NP_005535 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:250-1:1500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human IRS1 pS307 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. |
| Reactivity: | Bovine,Chicken,Dog,Human,Monkey,Mouse,Pig,Rat |
| Application image: |  |
| Application image note: | Western blot using IRS1 (phospho S307) polyclonal antibody (Cat # PAB10078) shows detection of a band at ~180 KDa believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/mL of anisomysin for 30 min. The pronounced staining of the band at 180 KDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competedout by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5%BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-RabbitIgG [H&L] MX for 45 min at room temperature (800 nmchannel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Salicylic acid reverses phorbol 12-myristate-13-acetate (PMA)- and tumor necrosis factor alpha (TNFalpha)-induced insulin receptor substrate 1 (IRS1) serine 307 phosphorylation and insulin resistance in human embryonic kidney 293 (HEK293) cells.Jiang G, Dallas-Yang Q, Liu F, Moller DE, Zhang BB. J Biol Chem. 2003 Jan 3;278(1):180-6. Epub 2002 Oct 29. |