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Data sheet of SH3BP2 (phospho S427) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about SH3BP2 (phospho S427) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10066 |
| Product name: | SH3BP2 (phospho S427) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of SH3BP2. |
| Gene id: | 6452 |
| Gene name: | SH3BP2 |
| Gene alias: | 3BP2|CRBM|CRPM|FLJ42079|RES4-23 |
| Gene description: | SH3-domain binding protein 2 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S427 of human SH3BP2. |
| Protein accession: | P78314;NP_003014;SH3BP2 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to phosphorylated human SH3BP2 protein at the pS427 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot analysis is shown using SH3BP2 (phospho S427) polyclonal antibody (Cat # PAB10066) to detect endogenous protein present in unstimulated human whole cell lysates. The bandas indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicatesa band of ~60 KDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilutionof the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The chaperone protein 14-3-3 interacts with 3BP2/SH3BP2 and regulates its adapter function.Foucault I, Liu YC, Bernard A, Deckert M. J Biol Chem. 2003 Feb 28;278(9):7146-53. Epub 2002 Dec 24. |