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Data sheet of GSK3A (phospho S21) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IF |
More info about GSK3A (phospho S21) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10054 |
| Product name: | GSK3A (phospho S21) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3A. |
| Gene id: | 2931 |
| Gene name: | GSK3A |
| Gene alias: | DKFZp686D0638 |
| Gene description: | glycogen synthase kinase 3 alpha |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S21 of human GSK3A. |
| Protein accession: | P49840;NP_063937 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. |
| Reactivity: | Chimpanzee,Human,Rat |
| Application image: |  |
| Application image note: | Western blot using GSK3A (phospho S21) polyclonal antibody (Cat # PAB10054) shows detection of a 52 KDa band corresponding to human GSK3A in various human derived 293T cell extracts. Cells were serum starved for 24 h. Lane 1 : control, Lane 2: treated with IGF-1 (100 ng/mL) for 20 min, Lane 3 : pre-treated with 10 uM PI3K inhibitor and treated with IGF-1 (100 ng/mL) for 20 min. Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when aninhibitor is added (Lane 3). Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocel followed by reaction with a 1:1,000 dilution of anti-GSK3A antibody. Signal was detected using standard techniques. Personal communication, Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada. |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Inhibition of glycogen synthase kinase 3 improves insulin action and glucose metabolism in human skeletal muscle.Nikoulina SE, Ciaraldi TP, Mudaliar S, Carter L, Johnson K, Henry RR. Diabetes. 2002 Jul;51(7):2190-8. |