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Data sheet of FANCC polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about FANCC polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10046 |
| Product name: | FANCC polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of FANCC. |
| Gene id: | 2176 |
| Gene name: | FANCC |
| Gene alias: | FA3|FAC|FACC|FLJ14675 |
| Gene description: | Fanconi anemia, complementation group C |
| Immunogen: | A synthetic peptide corresponding to amino acids 96-112 of human FANCC. |
| Protein accession: | NP_000127;Q00597 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:20000-1:80000) Western Blot (1:1000-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Human |
| Application image: |  |
| Application image note: | Western blot using FANCC polyclonal antibody (Cat # PAB10046) shows detection of a band at ~63 KDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate. The identity of the lower molecular weight band is unknown. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to1:1,500 in PBS supplemented with 1% normal goatserum and 0.1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution ofIRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made bycomparison to prestained MW markers (indicated at left). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The Fanconi anemia core complex associates with chromatin during S phase.Mi J, Kupfer GM. Blood. 2005 Jan 15;105(2):759-66. Epub 2004 Jul 15. |