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Data sheet of CCNB1 (phospho S126) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about CCNB1 (phospho S126) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10042 |
| Product name: | CCNB1 (phospho S126) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of CCNB1. |
| Gene id: | 891 |
| Gene name: | CCNB1 |
| Gene alias: | CCNB |
| Gene description: | cyclin B1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S126 of human CCNB1. |
| Protein accession: | P14635;NP_114172 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:50000) Western Blot (1:100-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to phosphorylated human Cyclin B1 protein at the pS126 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein. |
| Reactivity: | Chimpanzee,Dog,Human,Rat |
| Application image: |  |
| Application image note: | Western blot using CCNB1 (phospho S126) polyclonal antibody (Cat # PAB10042) shows detection of a band ~48 kDa corresponding to phosphorylated human CCNB1 (arrowheads) in various whole cell lysates. Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 ug of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel. Transfer occurred overnight at 4°C at 15 mAmps. The membrane was blocked with 5% non-fat dry milk in TTBS for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature. After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX was added for 1 h at room temperature. After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min. Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec Personnel Communication, Luca Cote, Temple U. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Regulation of activation-induced Fas (CD95/Apo-1) ligand expression in T cells by the cyclin B1/Cdk1 complex.Torgler R, Jakob S, Ontsouka E, Nachbur U, Mueller C, Green DR, Brunner T. J Biol Chem. 2004 Sep 3;279(36):37334-42. Epub 2004 Jun 23. |