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Data sheet of FBXO9 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Clawed frog,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IF |
More info about FBXO9 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10012 |
| Product name: | FBXO9 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of FBXO9. |
| Gene id: | 26268 |
| Gene name: | FBXO9 |
| Gene alias: | DKFZp434C0118|FBX9|KIAA0936|NY-REN-57|VCIA1|dJ341E18.2 |
| Gene description: | F-box protein 9 |
| Immunogen: | A synthetic peptide corresponding to amino acids 431-447 of human FBXO9. |
| Protein accession: | NP_036479(isoform1);NP_258441(isoform2);NP_258442(isoform3) |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends. |
| Reactivity: | Bovine,Chicken,Chimpanzee,Clawed frog,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using FBXO9 polyclonal antibody (Cat # PAB10012) shows detection of a band at ~100 KDa (arrowhead) believed to correspond to FBOX9 present in a MCF-7 whole cell lysate (Lane 1). Specific band reactivity is greatly diminished when theantibody is pre-incubated with the immunizing peptide (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,500. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red). IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | A family of mammalian F-box proteins.Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW. Curr Biol. 1999 Oct 21;9(20):1180-2. |