| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,ChIP,Dot-Pep,IF |
| Brand: | Abnova |
| Reference: | PAB0651 |
| Product name: | Histone H3 (K9ac) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (acetylated lysine 9). |
| Immunogen: | A synthetic peptide (conjugated with KLH) containing the acetylated lysine 9 (or [K9ac]) of human histone H3. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.1% BSA, 0.01% thimerosal) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | Immunofluorescence with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) . HeLa cells were stained with antibody directed against modified histone and with DAPI. Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS. The fixation step stabilises the morphology of the cells and permeabilizes membranes as well. (A) Cells were immunofluorescently labelled with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC. (B) Nuclei were DAPI stained to label specifically the DNA. In both cases, the nuclei are clearly stained. |
| Applications: | ELISA,ChIP,Dot-Pep,IF |
| Shipping condition: | Dry Ice |