|Product name:||Collagenase Type 3 (C. histolyticum)|
|Product description:||Lyophilized. Dialyzed. 0.22um filtered. Lower in secondary proteolytic contaminant activities but with typical collagenase activity.|
|Preparation method:||Native protein purified from Clostridium histolyticum|
|Recommend dilutions:||Collagenase Assay; Mammary primary cell isolation.|
|Storage instruction:||Store at 4Â°C on dry atmosphere. |
For primary cell isolation, culture and tissue dissociation: Reconstituted in balanced salt solutions (such as Hank's, Earle's and others) at concentrations from 0.05% to 0.5% (w/v). For enzymetic assay: Reconstituted in 0.05 M TES buffer with 0.36 mM calcium chloride, pH 7.5 at a concentration of 1.0 mg/ml.
|Shipping condition:||Blue Ice|