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His tag monoclonal antibody, clone HIS.H8

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Condition: New product

Data sheet of His tag monoclonal antibody, clone HIS.H8

Brand:	Abnova
Reference:	MAB9752
Product name:	His tag monoclonal antibody, clone HIS.H8
Product description:	Mouse monoclonal antibody raised against synthetic peptide of His tag.
Clone:	HIS.H8
Isotype:	IgG2b
Immunogen:	A synthetic peptide (conjugated with KLH) corresponding to His tag.
Form:	Liquid
Concentration:	1 mg/mL
Recommend dilutions:	Western Blot (1:1000-1:3000) Immunohistochemistry (1:500-1:2000) The optimal working dilution should be determined by the end user.
Storage buffer:	In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage instruction:	Store at -20Â°C. Aliquot to avoid repeated freezing and thawing.
Note:	This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type:	Primary antibodies
Host species:	Mouse
Specificity:	Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
Application image:
Application image note:	Immunofluorescence staining of HEK293 cells. A: untransfected control. B: His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752) (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
Applications:	IP,ELISA,IHC,Dot,IF,WB-Tr
Shipping condition:	Dry Ice