| Brand | Abnova | 
| Product type | Primary antibodies | 
| Reactivity | Human | 
| Host species | Mouse | 
| Applications | WB,ELISA | 
| Brand: | Abnova | 
| Reference: | MAB8304 | 
| Product name: | ESR1 monoclonal antibody, clone 5D4B1,5D4E3 | 
| Product description: | Mouse monoclonal antibody raised against partial recombinant ESR1. | 
| Clone: | 5D4B1,5D4E3 | 
| Isotype: | IgG2b | 
| Gene id: | 2099 | 
| Gene name: | ESR1 | 
| Gene alias: | DKFZp686N23123|ER|ESR|ESRA|Era|NR3A1 | 
| Gene description: | estrogen receptor 1 | 
| Immunogen: | Recombinant protein corresponding to truncated human ESR1. | 
| Form: | Liquid | 
| Recommend dilutions: | Western Blot (1:500-1:2000) ELISA (1:10000) The optimal working dilution should be determined by the end user.  | 
| Storage buffer: | In ascites | 
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing.  | 
| Product type: | Primary antibodies | 
| Host species: | Mouse | 
| Antigen species / target species: | Human | 
| Reactivity: | Human | 
| Application image: | ![]()  | 
| Application image note: | Western blot analysis using ESR1 monoclonal antibody, clone 5D4B1, 5D4E3 (Cat # MAB8304) against truncated ESR1 recombinant protein (Lane 1) and MCF-7 cell lysate (Lane 2). | 
| Applications: | WB,ELISA | 
| Shipping condition: | Dry Ice | 
| Publications: | Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance.Campbell RA, Bhat-Nakshatri P, Patel NM, Constantinidou D, Ali S, Nakshatri H. J Biol Chem. 2001 Mar 30;276(13):9817-24. Epub 2001 Jan 3.  |