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Data sheet of LGR4 monoclonal antibody, clone 6G8.B3.G5.C3
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human,Macaque |
| Host species | Mouse |
| Applications | WB,ELISA,IHC-P |
More info about LGR4 monoclonal antibody, clone 6G8.B3.G5.C3
| Brand: | Abnova |
| Reference: | MAB8166 |
| Product name: | LGR4 monoclonal antibody, clone 6G8.B3.G5.C3 |
| Product description: | Mouse monoclonal antibody raised against synthetic peptide of LGR4 . |
| Clone: | 6G8.B3.G5.C3 |
| Gene id: | 55366 |
| Gene name: | LGR4 |
| Gene alias: | GPR48 |
| Gene description: | leucine-rich repeat-containing G protein-coupled receptor 4 |
| Immunogen: | A synthetic peptide corresponding to internal region of human LGR4 . |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:20000-1:100000) Western Blot (1:500-1:3000) Immunohistochemistry (5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Human,Macaque |
| Application image: |  |
| Application image note: | Immunohistochemical staining of LGR4 monoclonal antibody, clone 6G8.B3.G5.C3 (Cat # MAB8166) was used diluted to 5 ug/mL to detect LGR4 staining at the membrane of cells in various human tissues. A. Brain cerebellum. B. Pancreas islet. Strongly positive staining is noted in subsets of cells within the islets of Langerhans. Moderately positive staiing was observed in Purkinje and Golgi neurons of the cerebellum, adrenal medulla, neuroendocrine cells, hepatocytes, lung macrophages, seminiferous tubules and Leydig cells of the testis. Faintly to moderately positive staining was also observed in cardiac myocytes and renal tubules, granulocytes, and subsets of lymphocytes. Some elastin background staining is noted. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA. |
| Applications: | WB,ELISA,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor.Loh ED, Broussard SR, Kolakowski LF. Biochem Biophys Res Commun. 2001 Apr 6;282(3):757-64. |