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| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse |
| Host species | Rat |
| Applications | WB,IP,IHC-Fr,ICC,IHC-P,IF,Flow Cyt |
| Brand: | Abnova |
| Reference: | MAB7100 |
| Product name: | Cd34 monoclonal antibody, clone MEC 14.7 |
| Product description: | Rat monoclonal antibody raised against Cd34. |
| Clone: | MEC 14.7 |
| Isotype: | IgG2a |
| Gene id: | 12490 |
| Gene name: | Cd34 |
| Gene alias: | AU040960 |
| Gene description: | CD34 antigen |
| Immunogen: | Full length mouse Cd34. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:250) Flow Cytometry (1 ug per million cells) Immunocytochemistry/Immunofluorescence (1:100-1:1000) Immunohistochemistry (1:250) Immunoprecipitation (1:10-1:500) The optimal working dilution should be determined by the end us |
| Storage buffer: | In PBS (0.02% sodium azide) |
| Storage instruction: | Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rat |
| Antigen species / target species: | Mouse |
| Specificity: | The reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries. |
| Reactivity: | Mouse |
| Application image: | ![]() |
| Application image note: | Immunocytochemistry with Cd34 monoclonal antibody, clone MEC 14.7 (Cat # MAB7100). Localization of Cd34 expression, LRCs, and K15 expression in murine hair follicles. (A) Cd34 expression in formalin-fixed, paraffin-embedded dorsal skin from an untreated Tg.AC mouse, showing membrane localization in the hair follicle. Arrow indicates Cd34-positive cells. (B) Cd34 expression in a murine telogen hair follicle. (C)Cd34 expression in a murine anagen hair follicle. Arrow indicates Cd34-positive keratinocytes in panels B and C. Scale bars: 100 mum. |
| Applications: | WB,IP,IHC-Fr,ICC,IHC-P,IF,Flow Cyt |
| Shipping condition: | Dry Ice |
| Publications: | Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, Tennant RW. J Invest Dermatol. 2003 Apr;120(4):501-11. |