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Data sheet of ITGB2 monoclonal antibody, clone IB4
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | Flow Cyt |
More info about ITGB2 monoclonal antibody, clone IB4
| Brand: | Abnova |
| Reference: | MAB6945 |
| Product name: | ITGB2 monoclonal antibody, clone IB4 |
| Product description: | Mouse monoclonal antibody raised against native ITGB2. |
| Clone: | IB4 |
| Isotype: | IgG2a |
| Gene id: | 3689 |
| Gene name: | ITGB2 |
| Gene alias: | CD18|LAD|LCAMB|LFA-1|MAC-1|MF17|MFI7 |
| Gene description: | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| Immunogen: | Native purified ITGB2 from human peripheral blood monocytes. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
| Storage instruction: | Store at 4°C. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | 5 X 105 cultured human HPB-MLT cells per tube were incubated 45 minutes on ice with 80 uL of ITGB2 monoclonal antibody, clone IB4 (Cat # MAB6945) at a concentration of 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive (67% net) with a mean shift of 0.92 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. |
| Applications: | Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N. J Cell Biol. 1994 Jul;126(2):529-37. |