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Data sheet of RB1 (phospho T821) monoclonal antibody, clone 24A7
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | WB,ELISA |
More info about RB1 (phospho T821) monoclonal antibody, clone 24A7
| Brand: | Abnova |
| Reference: | MAB6765 |
| Product name: | RB1 (phospho T821) monoclonal antibody, clone 24A7 |
| Product description: | Mouse monoclonal antibody raised against synthetic phosphopeptide of RB1. |
| Clone: | 24A7 |
| Isotype: | IgG1, kappa |
| Gene id: | 5925 |
| Gene name: | RB1 |
| Gene alias: | OSRC|RB|p105-Rb|pRb|pp110 |
| Gene description: | retinoblastoma 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T821 of human RB1. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.05% sodium azide) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to human RB1 phosphorylated at Thr821. It can detect endogenous levels of RB1 phosphorylated at Thr821 in most cell lines. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Specificity of the RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765) as demonstrated by western blotting. Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (Lane 1), Rb (S795A, lane 2) or Rb (T821A, lane 3) were immunoprecipitated with anti-Myc antibody and the precipitates were analyzed by western blotting with RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765). Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.Nevins JR. Science. 1992 Oct 16;258(5081):424-9. |