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Data sheet of Cd69 monoclonal antibody, clone H1.2F3 (SPRD)
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse |
| Host species | Hamster |
| Applications | IP,IHC-Fr,Flow Cyt |
More info about Cd69 monoclonal antibody, clone H1.2F3 (SPRD)
| Brand: | Abnova |
| Reference: | MAB5746 |
| Product name: | Cd69 monoclonal antibody, clone H1.2F3 (SPRD) |
| Product description: | Hamster monoclonal antibody raised against native Cd69. |
| Clone: | H1.2F3 |
| Isotype: | IgG, lambda |
| Gene id: | 12515 |
| Gene name: | Cd69 |
| Gene alias: | 5830438K24Rik|AI452015|AIM|VEA |
| Gene description: | CD69 antigen |
| Immunogen: | Native purified Cd69 from Y245 mouse dendritic epidermal T cell line. |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (0.2 ug/106 cells) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Conjugate tag: | SPRD |
| Product type: | Primary antibodies |
| Host species: | Hamster |
| Antigen species / target species: | Mouse |
| Specificity: | Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 KDa (unreduced). |
| Reactivity: | Mouse |
| Application image: |  |
| Application image note: | Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or Cd69 monoclonal antibody, clone H1.2F3 (FITC) (Cat # MAB5742). Large, activated cells were then gated and analyzed on a FACScanâ„¢ flow cytometer (BDIS, San Jose, CA). |
| Applications: | IP,IHC-Fr,Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation.Yokoyama WM, Maxfield SR, Shevach EM. Immunol Rev. 1989 Jun;109:153-76. |