| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | Flow Cyt |
| Brand: | Abnova |
| Reference: | MAB5024 |
| Product name: | HLA-Class I monoclonal antibody, clone MEM-147 (PE) |
| Product description: | Mouse monoclonal antibody raised against native HLA-Class I. |
| Clone: | MEM-147 |
| Isotype: | IgG1 |
| Immunogen: | Native purified HLA-Class I from PHA-activated peripheral blood lymphocytes. |
| Form: | Liquid |
| Concentration: | 0.1 mg/mL |
| Recommend dilutions: | Flow Cytometry (5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.2% BSA, 0.09% sodium azide) |
| Storage instruction: | Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Conjugate tag: | PE |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Specificity: | This antibody reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blot). This antibody is positive in Western blot (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. |
| Reactivity: | Human |
| Applications: | Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody.Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P. Immunogenetics. 1999 Apr;49(4):312-20. |