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| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Mouse |
| Applications | IP,ELISA,Dot,IF,MeDIP,Flow Cyt |
| Brand: | Abnova |
| Reference: | MAB4952 |
| Product name: | 5-mC monoclonal antibody, clone b |
| Product description: | Mouse monoclonal antibody raised against 5-methyl Cytosine. |
| Clone: | b |
| Isotype: | IgG1 |
| Immunogen: | Ovalbumine conjugated with 5-methyl Cytosine. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (0.1-0.3 ug/mL) Dot Blot (1-2 ug/mL) Immunofluorescence (1:100) Flow Cytometry (1-2 ug/mL) Methylated DNA Immunoprecipitation (1-5 ug/IP) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS |
| Storage instruction: | Aliquot and store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Reactivity: | Human,Mouse |
| Application image: | ![]() |
| Application image note: | Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952). A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um. B. Enlarged picture corresponding to a region from the left panel (as indicated in A). C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B). |
| Applications: | IP,ELISA,Dot,IF,MeDIP,Flow Cyt |
| Shipping condition: | Dry Ice |