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Data sheet of H3K27me2/3 monoclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | ChIP,Dot,WB-Ce,IF |
More info about H3K27me2/3 monoclonal antibody
| Brand: | Abnova |
| Reference: | MAB4947 |
| Product name: | H3K27me2/3 monoclonal antibody |
| Product description: | Mouse monoclonal antibody raised against H3K27me2/3. |
| Immunogen: | The region of the histone H3 containing the methylated lysine 27 (H3K27me2/3). |
| Form: | Liquid |
| Recommend dilutions: | Dot Blot (1:300) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Dot Blot is performed to determine the cross-reactivity of H3K27me2/3 monoclonal antibody (Cat # MAB4947) with other histone modifications. Other histone modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of two other lysines of H3 (K9 and K36). To determine the cross-reactivity, 0.2 to 100 pmol of peptide containing the respective histone modifications are spotted on a membrane. The antibody is used at a dilution of 1 : 300. The antibody recognizes di- and trimethylation of lysine 27 on histone H3. |
| Applications: | ChIP,Dot,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Tips in analyzing antibodies directed against specific histone tail modifications.Sarma K, Nishioka K, Reinberg D. Methods Enzymol. 2004;376:255-69. |