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| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | WB,IHC-P,Flow Cyt |
| Brand: | Abnova |
| Reference: | MAB3835 |
| Product name: | CD34 monoclonal antibody, clone 4H11[APG] |
| Product description: | Mouse monoclonal antibody raised against native CD34. |
| Clone: | 4H11[APG] |
| Isotype: | IgG1 |
| Gene id: | 947 |
| Gene name: | CD34 |
| Gene alias: | - |
| Gene description: | CD34 molecule |
| Immunogen: | Native purified CD34 from permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia. |
| Form: | Liquid |
| Concentration: | 1 mg/mL |
| Recommend dilutions: | Flow Cytometry (2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL) Western Blot (2 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.4 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. Do not freeze. Avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody reacts with Class III epitope on CD34 (Mucosialin), a 110-115 KDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line. |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | Surface staining of Kg-1a (human acute myelogenous leukemia cell line) with CD34 monoclonal antibody, clone 4H11[APG] (Cat # MAB3835) - FITC. Total viable cells were used for analysis. |
| Applications: | WB,IHC-P,Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution.Dao MA, Arevalo J, Nolta JA. Blood. 2003 Jan 1;101(1):112-8. Epub 2002 Jul 25. |