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Data sheet of ZAP70 monoclonal antibody, clone ZAP-03
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | ICC,WB-Ce,Flow Cyt |
More info about ZAP70 monoclonal antibody, clone ZAP-03
| Brand: | Abnova |
| Reference: | MAB3664 |
| Product name: | ZAP70 monoclonal antibody, clone ZAP-03 |
| Product description: | Mouse monoclonal antibody raised against partial recombinant ZAP70. |
| Clone: | ZAP-03 |
| Isotype: | IgG1 |
| Gene id: | 7535 |
| Gene name: | ZAP70 |
| Gene alias: | FLJ17670|FLJ17679|SRK|STD|TZK|ZAP-70 |
| Gene description: | zeta-chain (TCR) associated protein kinase 70kDa |
| Immunogen: | Recombinant His fusion protein corresponding to 160 amino acids of human ZAP70. |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (2-5 ug/mL) Western Blot (0.5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.4 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody reacts with ZAP70, a 70 KDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line). Lane 1, 2, 3, 4 : Immunostaining with dilution range of ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664) ; 4 ug/mL (1), 2 ug/mL (2), 1 ug/mL (3), 0.5 ug/mL (4). Lane 5 : Immunostaining with anti-ZAP70 comparative antibody. Lane 6 : Immunostaining with Isotype mouse IgG1 control. |
| Applications: | ICC,WB-Ce,Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells.Rajagopalan S, Long EO. J Exp Med. 1999 Apr 5;189(7):1093-100. |