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Data sheet of CD59 monoclonal antibody, clone MEM-43/5
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Mouse |
| Applications | WB,IP,IHC-P,Flow Cyt |
More info about CD59 monoclonal antibody, clone MEM-43/5
| Brand: | Abnova |
| Reference: | MAB3628 |
| Product name: | CD59 monoclonal antibody, clone MEM-43/5 |
| Product description: | Mouse monoclonal antibody raised against native CD59. |
| Clone: | MEM-43/5 |
| Isotype: | IgG2b |
| Gene id: | 966 |
| Gene name: | CD59 |
| Gene alias: | 16.3A5|1F5|EJ16|EJ30|EL32|FLJ38134|FLJ92039|G344|HRF-20|HRF20|MAC-IP|MACIF|MEM43|MGC2354|MIC11|MIN1|MIN2|MIN3|MIRL|MSK21|p18-20 |
| Gene description: | CD59 molecule, complement regulatory protein |
| Immunogen: | Native Thymocytes and T lymphocytes. |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (1 ug/mL) Western Blot (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.4 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 KDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. This antibody does not compete with most other CD59 antibodies. |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL (human peripheral blood T cell leukemia cell line) using CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628). Lane 1 : Original cell lysate. Lane 2 : Material immunoprecipitated with CD59 monoclonal antibody, clone MEM-43/5 (Cat # MAB3628). |
| Applications: | WB,IP,IHC-P,Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation.Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE. J Immunol. 1994 Sep 15;153(6):2444-56. |