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Drosophila VSVG NJ Recombinant Protein View larger

Drosophila VSVG NJ Recombinant Protein

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Data sheet of Drosophila VSVG NJ Recombinant Protein

BrandProteoGenix
Product typeProteins
Origin speciesFruit fry
Host speciesInsect

More info about Drosophila VSVG NJ Recombinant Protein

Proteogenix reference: PX-P2099-10
Protein delivered with tag?: Yes
Size: 10 ug
Product name: Drosophila VSVG NJ Recombinant Protein
Fragment type: Full-length
Expression system: Eukaryotic expression
Molecular weight with tag if any: 59.11kDa
Purity estimated: 0.95
Protein accession: NP_476656.1
Spec:entrez geneid: 33271
Spec:ncbi gene aliases: CG3966, NINAA, Dmel\CG3966, ninA, NinaA
Spec:swissprotid: P15425
Ncbi reference: NP_476656.1
Uniprot id: P15425
Uniprot link: http://www.uniprot.org/uniprot/P15425
Aliases / synonyms: VSVG, neither inactivation nor afterpotential A, Peptidyl-prolyl cis-trans isomerase, rhodopsin-specific isozyme, PPIase,Rotamase
Protein sequence (w/o tag): MKSLLNRIILCSAFLAVASGLSFTVTSRIYMDVKHNKKPVGRITFGLFGKLAPKTVANFRHICLRGINGTSYVGSRFHRVVDRFLVQGGDIVNGDGTGSISIYGDYFPDEDKALAVEHNRPGYLGMANRGPDTNGCQFYVTTVGAKWLDGKHTVFGKVLEGMDTIYAIEDVKTDTDDFPVEPVVISNCGEIPTEQFEFYPDDFNILGWIKAAGLPVTSSFCVLLIFHYFFRQLNMYCGGDYKDDDDK
Form: Frozen
Buffer: PBS, pH 7.5
Storage condition: 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
Delivery condition: Dry Ice
Delivery lead time in business days: 10-25
Image: PX-P2099.jpg
Description: Peptidyl-prolyl cis-trans isomerase, rhodopsin-specific isozyme also known as PPIase or Rotamase is a part of the cyclophillin-type PPIase family. The main role of the PPIases is the acceleration of the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and it Acts on the folding of rhodopsin RH1 and RH2 (but not RH3) and is required for visual transduction.
Publications: 1: Lenhard T, Reiländer H. Engineering the folding pathway of insect cells: generation of a stably transformed insect cell line showing improved folding of a recombinant membrane protein. Biochem Biophys Res Commun. 1997 Sep 29;238(3):823-30. PubMed PMID: 9325175. 2: Hemenway C, Heitman J. Proline isomerases in microorganisms and small eukaryotes. Ann N Y Acad Sci. 1993 Nov 30;696:38-46. Review. PubMed PMID: 8109845. 3: Larrivee DC, Conrad SK, Stephenson RS, Pak WL. Mutation that selectively affects rhodopsin concentration in the peripheral photoreceptors of Drosophila melanogaster. J Gen Physiol. 1981 Nov;78(5):521-45. PubMed PMID: 6796648; PubMed Central PMCID: PMC2228638. 4: Pak WL, Conrad SK, Kremer NE, Larrivee DC, Schinz RH, Wong F. Photoreceptor function. Basic Life Sci. 1980;16:331-46. PubMed PMID: 6779798. 5: Schneuwly S, Shortridge RD, Larrivee DC, Ono T, Ozaki M, Pak WL. Drosophila ninaA gene encodes an eye-specific cyclophilin (cyclosporine A binding protein). Proc Natl Acad Sci U S A. 1989 Jul;86(14):5390-4. PubMed PMID: 2664782; PubMed Central PMCID: PMC297628. 6: Shieh BH, Stamnes MA, Seavello S, Harris GL, Zuker CS. The ninaA gene required for visual transduction in Drosophila encodes a homologue of cyclosporin A-binding protein. Nature. 1989 Mar 2;338(6210):67-70. PubMed PMID: 2493138. 7: Webel R, Menon I, O'Tousa JE, Colley NJ. Role of asparagine-linked oligosaccharides in rhodopsin maturation and association with its molecular chaperone, NinaA. J Biol Chem. 2000 Aug 11;275(32):24752-9. PubMed PMID: 10811808. 8: Kurada P, Tonini TD, Serikaku MA, Piccini JP, O'Tousa JE. Rhodopsin maturation antagonized by dominant rhodopsin mutants. Vis Neurosci. 1998 Jul-Aug;15(4):693-700. PubMed PMID: 9682871. 9: Bentrop J, Schwab K, Pak WL, Paulsen R. Site-directed mutagenesis of highly conserved amino acids in the first cytoplasmic loop of Drosophila Rh1 opsin blocks rhodopsin synthesis in the nascent state. EMBO J. 1997 Apr 1;16(7):1600-9. PubMed PMID: 9130705; PubMed Central PMCID: PMC1169764. 10: Ondek B, Hardy RW, Baker EK, Stamnes MA, Shieh BH, Zuker CS. Genetic dissection of cyclophilin function. Saturation mutagenesis of the Drosophila cyclophilin homolog ninaA. J Biol Chem. 1992 Aug 15;267(23):16460-6. PubMed PMID: 1644830. 11: Zuker CS, Mismer D, Hardy R, Rubin GM. Ectopic expression of a minor Drosophila opsin in the major photoreceptor cell class: distinguishing the role of primary receptor and cellular context. Cell. 1988 May 6;53(3):475-82. PubMed PMID: 2966681. 12: Fouillet A, Levet C, Virgone A, Robin M, Dourlen P, Rieusset J, Belaidi E, Ovize M, Touret M, Nataf S, Mollereau B. ER stress inhibits neuronal death by promoting autophagy. Autophagy. 2012 Jun;8(6):915-26. doi: 10.4161/auto.19716. Epub 2012 Jun 1. PubMed PMID: 22660271; PubMed Central PMCID: PMC3427257. 13: Mitra A, Chinchore Y, Kinser R, Dolph PJ. Characterization of two dominant alleles of the major rhodopsin-encoding gene ninaE in Drosophila. Mol Vis. 2011;17:3224-33. Epub 2011 Dec 14. PubMed PMID: 22194648; PubMed Central PMCID: PMC3244490. 14: Ahmad ST, Natochin M, Barren B, Artemyev NO, O'Tousa JE. Heterologous expression of bovine rhodopsin in Drosophila photoreceptor cells. Invest Ophthalmol Vis Sci. 2006 Sep;47(9):3722-8. PubMed PMID: 16936079. 15: Ozaki K, Nagatani H, Ozaki M, Tokunaga F. Maturation of major Drosophila rhodopsin, ninaE, requires chromophore 3-hydroxyretinal. Neuron. 1993 Jun;10(6):1113-9. PubMed PMID: 8318232.

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